Impact of DNA extraction, PCR amplification, sequencing, and bioinformatic analysis on food-associated mock communities using PacBio long-read amplicon sequencing

Abstract Background Long-read 16S rRNA gene amplicon sequencing has a high potential for characterizing food-associated microbiomes. The advantage results from sequencing the full-length (1,500 bp) gene, enabling taxonomic resolution at species level. Here we present a benchmarking study using mock...

Volledige beschrijving

Bibliografische gegevens

Hoofdauteurs:	Mareike Baer, Lisa Höppe, Waldemar Seel, André Lipski
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	BMC 2024-12-01
Reeks:	BMC Microbiology
Onderwerpen:	PacBio sequencing Full-length 16S rRNA gene Long-read sequencing Amplicon sequencing Mock community Food microbiota
Online toegang:	https://doi.org/10.1186/s12866-024-03677-8
Tags:	Voeg label toe Geen labels, Wees de eerste die dit record labelt!