A CRISPR-Cas9 library to target putative redundant gene sets facilitates their functional exploration in grain development in rice

Abstract Advent of CRISPR-Cas9 library approach has revolutionized the field of high throughput targeted mutagenesis in plants. By identifying an sgRNA spacer that can target multiple paralogous genes in a genome, higher-order knockout plants can be developed. Using this concept, we developed ten CR...

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Detalhes bibliográficos

Principais autores:	Banita Yadav, Shaswati Sardar, Anil Yadav, Annapurna Kumari, Mohini Gautam, Rushil Mandlik, Simran Arora, Shailesh Kumar, Pawan Kumar Jewaria, Humira Sonah, Rupesh Deshmukh, Viswanathan Chinnusamy, Hasthi Ram
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2025-11-01
coleção:	BMC Plant Biology
Assuntos:	Rice CRISPR-Cas9 library Genome-editing Higher-order knockout Grain Functional genomics
Acesso em linha:	https://doi.org/10.1186/s12870-025-07769-z
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