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Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice

Brain endothelial cells are the major building block of the blood-brain barrier. To study the role of brain endothelial cells in vitro, the isolation of primary cells is of critical value. Here, we describe a protocol in which vessel fragments are isolated from adult mice. After density centrifugati...

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Auteurs principaux: Julian Assmann, Kristin Müller, Jan Wenzel, Thomas Walther, Josefine Brands, Peter Thornton, Stuart Allan, Markus Schwaninger
Format: Artigo
Langue:Inglês
Publié: Bio-protocol LLC 2017-05-01
Collection:Bio-Protocol
Accès en ligne:https://bio-protocol.org/en/bpdetail?id=2294&type=0
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