Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems in Mammalian Cells

CRISPR-Cas9 is quickly revolutionizing the way we approach gene therapy. CRISPR-Cas9 is a complexed, two-component system using a short guide RNA (gRNA) sequence to direct the Cas9 endonuclease to the target site. Modifying the gRNA independent of the Cas9 protein confers ease and flexibility to imp...

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Principais autores:	Daniel Allen, Michael Rosenberg, Ayal Hendel
格式:	Artigo
語言:	Inglês
出版:	Frontiers Media S.A. 2021-01-01
叢編:	Frontiers in Genome Editing
主題:	CRISPR-Cas9 engineered nuclease gRNA chemical modifications genome editing gene therapy
在線閱讀:	https://www.frontiersin.org/articles/10.3389/fgeed.2020.617910/full
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