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Measuring Nucleosome Assembly Activity in vitro with the Nucleosome Assembly and Quantification (NAQ) Assay

Nucleosomes organize the eukaryotic genome into chromatin. In cells, nucleosome assembly relies on the activity of histone chaperones, proteins with high binding affinity to histones. At least a subset of histone chaperones promotes histone deposition in vivo. However, it has been challenging to cha...

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Autori principali: Francesca Mattiroli, Yajie Gu, Karolin Luger
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-protocol LLC 2018-02-01
Serie:Bio-Protocol
Accesso online:https://bio-protocol.org/en/bpdetail?id=2714&type=0
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