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Protocol for deriving proximity, affinity, and stoichiometry of protein interactions using image-based quantitative two-hybrid FRET

Summary: Two-hybrid Förster resonance energy transfer (FRET) provides proximity, affinity, and stoichiometry information in binding interactions. We present an image-based approach that surpasses traditional two-hybrid FRET assays in precision and robustness. We outline instrument setup and image ac...

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Principais autores: Colin Feldmann, Michael Schänzler, Manu Ben-Johny, Christian Wahl-Schott
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2023-09-01
Serier:STAR Protocols
Fag:
Microscopy
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166723004264
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