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Excision of Nucleopolyhedrovirus Form Transgenic Silkworm Using the CRISPR/Cas9 System

The CRISPR/Cas9-mediated genome engineering has been shown to efficiently suppress infection by disrupting genes of the pathogen. We recently constructed transgenic lines expressing CRISPR/Cas9 and the double sgRNA target Bombyx mori nucleopolyhedrovirus (BmNPV) immediate early-1 (ie-1) gene in the...

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Detalles Bibliográficos
Principais autores: Zhanqi Dong, Feifan Dong, Xinbo Yu, Liang Huang, Yaming Jiang, Zhigang Hu, Peng Chen, Cheng Lu, Minhui Pan
Formato: Artigo
Idioma:Inglês
Publicado: Frontiers Media S.A. 2018-02-01
Series:Frontiers in Microbiology
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Acceso en liña:http://journal.frontiersin.org/article/10.3389/fmicb.2018.00209/full
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