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sgRNA Knock-in Mouse Provides an Alternative Approach for In Vivo Genetic Modification

Functional genomics in a mammalian model such as mice is fundamental for understanding human biology. The CRISPR/Cas9 system dramatically changed the tempo of obtaining genetic mouse models due to high efficiency. However, experimental evidence for the establishment of sgRNA knock-in animals and ana...

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Principais autores: Lichen Zhang, Wushan Li, Zhuangzhuang Liu, Yang Liu, Zhilong Liu, Yanrong Gu, Le He, Binhui Zhou, Tianhan Li, Tianzhu Chao, Yinming Liang, Liaoxun Lu
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2022-01-01
coleção:Frontiers in Cell and Developmental Biology
Assuntos:
sgRNA knock-in
mouse model
genetic modification
Gfi1
neutrophil
Acesso em linha:https://www.frontiersin.org/articles/10.3389/fcell.2021.769673/full
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