Establishment of an Integrated CRISPR/Cas9 Plasmid System for Simple and Efficient Genome Editing in Medaka In Vitro and In Vivo

Although CRISPR/Cas9 has been used in gene manipulation of several fish species in vivo, its application in fish cultured cells is still challenged and limited. In this study, we established an integrated CRISPR/Cas9 plasmid system and evaluated its efficiency of gene knock-out or knock-in at a spec...

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Ngā kaituhi matua:	Zeming Zhang, Jie Wang, Jianeng Li, Xiang Liu, Lei Liu, Changle Zhao, Wenjing Tao, Deshou Wang, Jing Wei
Hōputu:	Artigo
Reo:	Inglês
I whakaputaina:	MDPI AG 2023-02-01
Rangatū:	Biology
Ngā marau:	CRISPR/Cas9 knock-out knock-in fish cultured cells U6 promoter medaka
Urunga tuihono:	https://www.mdpi.com/2079-7737/12/2/336
Ngā Tūtohu:	Tāpirihia he Tūtohu Kāore He Tūtohu, Me noho koe te mea tuatahi ki te tūtohu i tēnei pūkete!