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Site-directed mutation of a laccase from Thermus thermophilus: Effect on the activity profile

A site-directed mutant R453T of a laccase from Thermus thermophilus HB27 (Tth-laccase) was constructed in order to investigate the effect on laccase catalytic properties. The mutated gene was cloned and overexpressed in Escherichia coli. Nickel-affinity purification was achieved and followed by...

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Liu Xin, Tian Meng, Liu Kewu
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: University of Belgrade, University of Novi Sad 2012-01-01
Σειρά:Archives of Biological Sciences
Θέματα:
Διαθέσιμο Online:http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641204515L.pdf
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