Site-directed mutation of a laccase from Thermus thermophilus: Effect on the activity profile
A site-directed mutant R453T of a laccase from Thermus thermophilus HB27 (Tth-laccase) was constructed in order to investigate the effect on laccase catalytic properties. The mutated gene was cloned and overexpressed in Escherichia coli. Nickel-affinity purification was achieved and followed by...
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| Principais autores: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
University of Belgrade, University of Novi Sad
2012-01-01
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| coleção: | Archives of Biological Sciences |
| Assuntos: | |
| Acesso em linha: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641204515L.pdf |
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