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The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History

Self-cleaving affinity tags, based on engineered intein protein domains, have been touted as a universal single step purification platform for tagless non-mAb proteins. These approaches provide all of the power and flexibility of tag-based affinity methods, but deliver a tagless target protein suita...

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Principais autores: Sai Vivek Prabhala, Izabela Gierach, David W. Wood
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2022-04-01
Serija:Frontiers in Molecular Biosciences
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Online dostop:https://www.frontiersin.org/articles/10.3389/fmolb.2022.857566/full
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