Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis

Phytase efficiently hydrolyzes phytate to phosphate; thus, it is widely used to increase phosphorus availability in animal feeds and reduce phosphorus pollution through excretion. Phytase is easily inactivated during feed pelleting at high temperature, and sufficient thermostability of phytase is es...

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Detalhes bibliográficos

Principais autores:	Hongguan Xing, Pingping Wang, Xing Yan, Yi Yang, Xinliang Li, Rui Liu, Zhihua Zhou
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2023-03-01
coleção:	Frontiers in Bioengineering and Biotechnology
Assuntos:	phytase thermostability error-prone PCR directed evolution disulfide bond
Acesso em linha:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1167530/full
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