Single step production of Cas9 mRNA for zygote injection
Production of Cas9 mRNA in vitro typically requires the addition of a 5´ cap and 3´ polyadenylation. A plasmid was constructed that harbored the T7 promoter followed by the EMCV IRES and a Cas9 coding region. We hypothesized that the use of the metastasis associated lung adenocarcinoma transcript 1...
Збережено в:
| Автори: | , , , , , , , , |
|---|---|
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Taylor & Francis Group
2018-03-01
|
| Серія: | BioTechniques |
| Предмети: | |
| Онлайн доступ: | https://www.future-science.com/doi/10.2144/btn-2017-0116 |
| Теги: |
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|