Single step production of Cas9 mRNA for zygote injection
Production of Cas9 mRNA in vitro typically requires the addition of a 5´ cap and 3´ polyadenylation. A plasmid was constructed that harbored the T7 promoter followed by the EMCV IRES and a Cas9 coding region. We hypothesized that the use of the metastasis associated lung adenocarcinoma transcript 1...
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| Principais autores: | , , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Taylor & Francis Group
2018-03-01
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| Serier: | BioTechniques |
| Fag: | |
| Online adgang: | https://www.future-science.com/doi/10.2144/btn-2017-0116 |
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