A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids
Summary: Patient-derived organoids (PDOs) recapitulate the cellular heterogeneity of the original colorectal tumor tissue. Here, we describe a protocol to generate genetically modified PDOs to investigate cancer stem cells. This protocol uses the CRISPR-Cas9 system to knock-in the IRES-EGFP-P2A-iCas...
Guardat en:
| Autors principals: | , , , , |
|---|---|
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2021-12-01
|
| Col·lecció: | STAR Protocols |
| Matèries: | |
| Accés en línia: | http://www.sciencedirect.com/science/article/pii/S266616672100486X |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|