Multiplex real-time PCR with high-resolution melting analysis for rapid identification of carbapenem and colistin resistance genes in clinical Enterobacterales isolates

Abstract Conventional susceptibility testing requires 18–48 h and often delays therapy, whereas existing molecular assays are costly and limited. Real-time PCR’s ubiquity in hospitals offers a rapid multiplex screening platform. We established a single-tube multiplex real-time PCR assay coupled with...

Description complète

Détails bibliographiques

Auteurs principaux:	Sirirat Luk-In, Kamonrat Phopin, Sasina Bangmuangngam, Tanittha Chatsuwan, Dhammika Leshan Wannigama, Aye Mya Sithu Shein, Ratana Lawung, Tanawut Tantimongcolwat
Format:	Artigo
Langue:	Inglês
Publié:	Nature Portfolio 2026-03-01
Collection:	Scientific Reports
Sujets:	Carbapenemase gene mcr Enterobacterales Multiplex detection assay
Accès en ligne:	https://doi.org/10.1038/s41598-026-41530-2
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!