Multiplex real-time PCR with high-resolution melting analysis for rapid identification of carbapenem and colistin resistance genes in clinical Enterobacterales isolates

Abstract Conventional susceptibility testing requires 18–48 h and often delays therapy, whereas existing molecular assays are costly and limited. Real-time PCR’s ubiquity in hospitals offers a rapid multiplex screening platform. We established a single-tube multiplex real-time PCR assay coupled with...

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Principais autores:	Sirirat Luk-In, Kamonrat Phopin, Sasina Bangmuangngam, Tanittha Chatsuwan, Dhammika Leshan Wannigama, Aye Mya Sithu Shein, Ratana Lawung, Tanawut Tantimongcolwat
Format:	Artigo
Jezik:	Inglês
Izdano:	Nature Portfolio 2026-03-01
Serija:	Scientific Reports
Teme:	Carbapenemase gene mcr Enterobacterales Multiplex detection assay
Online dostop:	https://doi.org/10.1038/s41598-026-41530-2
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