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Global Run-On sequencing to measure nascent transcription in C. elegans

Summary: Global Run-On sequencing (GRO-seq) is one of the most sensitive techniques to detect nascent transcription from RNA polymerase (Pol) at a genome-wide level. The protocol incorporates labeled ribonucleotides into nascent RNAs from Pol I, II, and III. We have adapted the GRO-seq protocol to t...

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Principais autores: Piergiuseppe Quarato, Germano Cecere
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021-12-01
Serier:STAR Protocols
Fag:
Cell separation/fractionation
Gene Expression
Genomics
Model Organisms
Molecular Biology
RNAseq
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166721006973
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