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Application of high-throughput 5′P sequencing for the study of co-translational mRNA decay

Summary: mRNA degradation is connected to the translation process up to the degree that 5′-3′ mRNA degradation follows the last translating ribosome. To study 5′-3′co-translational mRNA decay and the associated ribosome dynamics, here we present an improved high-throughput 5′P degradome RNA sequenci...

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Bibliografiske detaljer
Principais autores: Yujie Zhang, Vicent Pelechano
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021-06-01
Serier:STAR Protocols
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Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166721001544
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