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Protein Pull-down Assay Using HiBiT-tag-dependent Luciferase Activity Measurement

Co-immunoprecipitation or pull-down assays are frequently used to analyze protein–protein interactions. In these experiments, western blotting is commonly used to detect prey proteins. However, sensitivity and quantification problems remain in this detection system. Recently, the HiBiT-tag-dependent...

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Bibliografiske detaljer
Principais autores: Masashi Arakawa, Eiji Morita
Format: Artigo
Sprog:Inglês
Udgivet: Bio-protocol LLC 2023-03-01
Serier:Bio-Protocol
Online adgang:https://bio-protocol.org/en/bpdetail?id=4640&type=0
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