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Real-time protein unfolding: a method for determining the kinetics of native protein denaturation using a quantitative real-time thermocycler

Protein stability can be monitored by many different techniques. However, these protocols are often lengthy, consume large amounts of protein, and require expensive and specialized instruments. Here we present a new protocol to analyze protein unfolding kinetics using a quantified real-time thermocy...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Kyle K. Biggar, Neal J. Dawson, Kenneth B. Storey
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Taylor & Francis Group 2012-10-01
Cyfres:BioTechniques
Pynciau:
Mynediad Ar-lein:https://www.future-science.com/doi/10.2144/0000113922
Tagiau: Ychwanegu Tag
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