Real-time protein unfolding: a method for determining the kinetics of native protein denaturation using a quantitative real-time thermocycler
Protein stability can be monitored by many different techniques. However, these protocols are often lengthy, consume large amounts of protein, and require expensive and specialized instruments. Here we present a new protocol to analyze protein unfolding kinetics using a quantified real-time thermocy...
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| Автори: | , , |
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| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Taylor & Francis Group
2012-10-01
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| Серія: | BioTechniques |
| Предмети: | |
| Онлайн доступ: | https://www.future-science.com/doi/10.2144/0000113922 |
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