A katE katG double-knockout E. coli strain eliminates the risk of catalase contamination in recombinant proteins

Abstract Recombinant protein expression in E. coli is a key methodology for modern biomedical research. Typically, a polyhistidine-tagged (“His-tagged”) protein is purified using immobilized metal affinity chromatography (IMAC), achieving close to apparently homogenous target protein preparations. H...

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Podrobná bibliografie

Hlavní autoři:	Axel Tobias Scholz, Lucia Coppo, Edward Nolan, Michaella Hernandez, Xuan Wang, Pradeep Mishra, Robert Schnell, Zsuzsanna Anna Pató, Yifei Chen, Markus Dagnell, Attila Andor, Qing Cheng, Elias S. J. Arnér
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Springer 2026-04-01
Edice:	Applied Microbiology and Biotechnology
Témata:	Recombinant protein expression Escherichia coli (E. coli) Protein purification Hydrogen peroxide Catalase Peroxiredoxin
On-line přístup:	https://doi.org/10.1007/s00253-026-13820-2
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