A katE katG double-knockout E. coli strain eliminates the risk of catalase contamination in recombinant proteins

Abstract Recombinant protein expression in E. coli is a key methodology for modern biomedical research. Typically, a polyhistidine-tagged (“His-tagged”) protein is purified using immobilized metal affinity chromatography (IMAC), achieving close to apparently homogenous target protein preparations. H...

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Detalles Bibliográficos

Principais autores:	Axel Tobias Scholz, Lucia Coppo, Edward Nolan, Michaella Hernandez, Xuan Wang, Pradeep Mishra, Robert Schnell, Zsuzsanna Anna Pató, Yifei Chen, Markus Dagnell, Attila Andor, Qing Cheng, Elias S. J. Arnér
Formato:	Artigo
Idioma:	Inglês
Publicado:	Springer 2026-04-01
Series:	Applied Microbiology and Biotechnology
Assuntos:	Recombinant protein expression Escherichia coli (E. coli) Protein purification Hydrogen peroxide Catalase Peroxiredoxin
Acceso en liña:	https://doi.org/10.1007/s00253-026-13820-2
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