SpEDIT: A fast and efficient CRISPR/Cas9 method for fission yeast [version 1; peer review: 2 approved]
The CRISPR/Cas9 system allows scarless, marker-free genome editing. Current CRISPR/Cas9 systems for the fission yeast Schizosaccharomyces pombe rely on tedious and time-consuming cloning procedures to introduce a specific sgRNA target sequence into a Cas9-expressing plasmid. In addition, Cas9 endonu...
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| Principais autores: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Wellcome
2020-11-01
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| coleção: | Wellcome Open Research |
| Acesso em linha: | https://wellcomeopenresearch.org/articles/5-274/v1 |
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