Construction of B16 cell strain expressing CSDE1-EGFP using CRISPR-PITCH system
Objective To construct a melanoma cell strain expressing endotopic CSDE1-EGFP using new CRISPR-PITCH system, then to select the cell strain of low expression CSDE1 and high expression CSDE1. Methods The EGFP coding sequence was inserted into the last exon of the CSDE1 gene using a micro-homology-m...
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| Автор: | |
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| Формат: | Artigo |
| Мова: | Chinês |
| Опубліковано: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-03-01
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| Серія: | Jichu yixue yu linchuang |
| Предмети: | |
| Онлайн доступ: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a201384.pdf |
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