Construction of B16 cell strain expressing CSDE1-EGFP using CRISPR-PITCH system

Objective To construct a melanoma cell strain expressing endotopic CSDE1-EGFP using new CRISPR-PITCH system, then to select the cell strain of low expression CSDE1 and high expression CSDE1. Methods The EGFP coding sequence was inserted into the last exon of the CSDE1 gene using a micro-homology-m...

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Hlavní autor:	LIU Meng-yu, ZHOU Ya-bo, HUANG Bo, LYU Jia-di
Médium:	Artigo
Jazyk:	Chinês
Vydáno:	Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2021-03-01
Edice:	Jichu yixue yu linchuang
Témata:	crispr-pitch system|csde1|micro-homology-meditated end joining(mmej)|enhanced green fluorescence protein(egfp)
On-line přístup:	http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a201384.pdf
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