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Expression and purification of phage T7 ejection proteins for cryo-EM analysis

Summary: Bacteriophages of the Podoviridae family densely package their genomes into precursor capsids alongside internal virion proteins called ejection proteins. In phage T7 these proteins (gp14, gp15, and gp16) are ejected into the host envelope forming a DNA-ejectosome for genome delivery. Here,...

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Hauptverfasser: Nicholas A. Swanson, Ravi K. Lokareddy, Fenglin Li, Chun-feng Hou, Mikhail Pavlenok, Michael Niederweis, Gino Cingolani
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2021-12-01
Schriftenreihe:STAR Protocols
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Online-Zugang:http://www.sciencedirect.com/science/article/pii/S2666166721006663
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