Adeno-associated virus Bayes optimization CHO DP-12 Chinese hamster ovary cells Escherichia coli Gaussian processes Kolmogorov length scale N−1 perfusion PAT PEI Pareto optimization biopharmaceutical manufacturing bioprocess bioreactor bioreactor characterization biosensor biotherapeutics production cell culture cell culture model clonal cell population computational fluid dynamics continuous critical shear stress cryopreservation diabetes gene therapy hybrid modeling hydrodynamic gradients hydrodynamic stress inoculum train