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Preferential Protection of Domains II and III of Bacillus thuringiensis Cry1Aa Toxin by Brush Border Membrane Vesicles

The surface exposed Leucine 371 on loop 2 of domain II, in Cry1Aa toxin, was mutated to Lysine to generate the trypsin-sensitive mutant, L371K. Upon trypsin digestion L371K is cleaved into approximately 37 and 26 kDa fragments. These are separable on SDS-PAGE, but remain as a single molecule of 65 k...

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Detalhes bibliográficos
Publicado no:Revista Colombiana de Biotecnología
Main Authors: Syed-Rehan A. Hussain, Álvaro M. Flórez, Donald H. Dean, Óscar Alzate
Formato: Artigo
Idioma:Inglês
Publicado em: Universidad Nacional de Colombia 2010
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Acesso em linha:https://www.redalyc.org/articulo.oa?id=77617808002
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