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Rapid DNA extraction and PCR validation for direct detection of Listeria monocytogenes in raw milk

Objective. The aim of this study was to validate a method for detecting L. monocytogenes in raw milk.Materials and methods methods. The extraction procedure carried out using a chaotropic agent like NaI, toreduce fat in the sample to 0.2% w/v, which is the lowest limit for detection in the Gerber me...

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Vydáno v:Revista MVZ Córdoba
Hlavní autoři: Edith Burbano, Sara Sierra, Kirvis Torres, Marcela Mercado, Ana Carrascal, Raúl Poutou
Médium: Artigo
Jazyk:Inglês
Vydáno: Universidad de Córdoba 2006
Témata:
PCR
On-line přístup:https://www.redalyc.org/articulo.oa?id=69311105
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