Detection and quantification of Alternaria solani in tomato by real time PCR

A conventional and real-time PCR assays using SYBR Green for the detection and quantification of A. solani have been developed and validated. A primer set (ALP and ITS4) designed from the ITS region of A. linicola/ A. solani complex, yielded a 536 bp product when DNA from 38 isolates of A. solani we...

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Veröffentlicht in:Journal of Horticultural Sciences
Hauptverfasser: P. Chowdappa, B.J. Nirmal Kumar, B. Reddi Bhargavi, K.R. Hema, S.P. Mohan Kumar
Format: Artigo
Sprache:Inglês
Veröffentlicht: Society for Promotion of Horticulture 2014
Schlagworte:
Agrociencias
early blight
Real Time PCR
Alternaria solani
molecular diagnosis
Online Zugang:https://www.redalyc.org/articulo.oa?id=577061808014
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