Lipofection improves gene targeting efficiency in E14 TG2a mouse embryonic stem cells

Electroporation has been the method of election for transfection of murine embryonic stem cells for over 15 years; however, it is a time consuming protocol because it requires large amounts of DNA and cells, as well as expensive and delicate equipment. Lipofection is a transfection method that requi...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:Veterinaria México
Main Authors: Sandra M. López-Heydeck, Marcos Cajero-Juárez, Rogelio A. Alonso-Morales, José S. Martínez-Castañeda, José F. Robles-González, Alberto Barbabosa-Pliego, Juan C. Vázquez-Chagoyán
Formato: Artigo
Idioma:Inglês
Publicado em: Universidad Nacional Autónoma de México 2009
Assuntos:
Veterinaria
LIPOFECTION
TRANSFECTION
GENE TARGETING
ELECTROPORATION
HOMOLOGOUS RECOMBINATION
Acesso em linha:https://www.redalyc.org/articulo.oa?id=42311566009
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados