Radiation dose determines the method for quantification of DNA double strand breaks

Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX ( γ H2AX). Immuno fluorescent staining visualizes formation of γ H2AX foci, allowing their quanti fi cation. This method, as opposed to Western blot assay and Flow cytometry, provi...

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Bibliografiske detaljer
Udgivet i:Anais da Academia Brasileira de Ciências
Main Authors: TANJA BULAT, OTILIJA KETA, LELA KORIĆANAC, JELENA ŽAKULA, IVAN PETROVIĆ, ALEKSANDRA RISTIĆ-FIRA, DANIJELA TODOROVIĆ
Format: Artigo
Sprog:Inglês
Udgivet: Academia Brasileira de Ciências 2016
Fag:
Multidisciplinaria (Ciencias Naturales y Exactas)
γ H2AX
Western blot
ApoTome software
AxioImagerA1 microscope
immunofluorescence microscopy
Online adgang:https://www.redalyc.org/articulo.oa?id=32744812011
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