Purification, characterization and structural determination of chitinases produced by Moniliophthora perniciosa

The enzyme chitinase from Moniliophthora perniciosa the causative agent of the witches' broom disease in Theobroma cacao, was partially purified with ammonium sulfate and filtration by Sephacryl S-200 using sodium phosphate as an extraction buffer. Response surface methodology (RSM) was used to...

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Bibliografiske detaljer
Udgivet i:Anais da Academia Brasileira de Ciências
Main Authors: Rafaela S. Galante, Alex G. Taranto, Maria G.B. Koblitz, Aristóteles Góes-Neto, Carlos P. Pirovani, Júlio C.M. Cascardo, Sandra H. Cruz, Gonçalo A.G. Pereira, Sandra A. de Assis
Format: Artigo
Sprog:Inglês
Udgivet: Academia Brasileira de Ciências 2012
Fag:
Multidisciplinaria (Ciencias Naturales y Exactas)
Chitinase
isoenzymes
purification
3D structure
heat stability
Online adgang:https://www.redalyc.org/articulo.oa?id=32722628003
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