Assessment of DNA extraction methods for detection of arbuscular mycorrhizal fungi in plant roots by nested-PCR

DNA extraction methods were evaluated for the yield and pu rity of DNA recovered from mycorrhized roots and whether the recovered DNA is suitable for amplification of arbuscular mycorrhizal (AM) fungal SSU rDNA. The DNeasy Plant Mini Kit an d three extraction buffers were used alone or in combinatio...

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Библиографические подробности
Опубликовано в: :Acta Scientiarum. Biological Sciences
Главные авторы: Abdala Gamby Diédhiou, Wardsson Lustrino Borges, Oumar Sadio, Sergio Miana de Faria
Формат: Artigo
Язык:Inglês
Опубликовано: Universidade Estadual de Maringá 2014
Предметы:
Biología
Keywords
TE buffer
DNA quality
activated charcoal
Online-ссылка:https://www.redalyc.org/articulo.oa?id=187132092008
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