Detection of Verrucomicrobia in a Pasture Soil by PCR-Mediated Amplification of 16S rRNA Genes

Oligonucleotide primers were designed and used to amplify, by PCR, partial 16S rRNA genes of members of the bacterial division Verrucomicrobia in DNA extracted from a pasture soil. By applying most-probable-number theory to the assay, verrucomicrobia appeared to contribute some 0.2% of the soil DNA....

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	O’Farrell, Katrina A., Janssen, Peter H.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	General Microbial Ecology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC99779/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10473454/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.9.4280-4284.1999
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