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Cloning of the Gene Encoding a Novel Thermostable α-Galactosidase from Thermus brockianus ITI360

An α-galactosidase gene from Thermus brockianus ITI360 was cloned, sequenced, and expressed in Escherichia coli, and the recombinant protein was purified. The gene, designated agaT, codes for a 476-residue polypeptide with a calculated molecular mass of 53,810 Da. The native structure of the recombi...

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Main Authors: Fridjonsson, Olafur, Watzlawick, Hildegard, Gehweiler, Axel, Rohrhirsch, Thilo, Mattes, Ralf
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 1999
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Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC99726/
https://ncbi.nlm.nih.gov/pubmed/10473401
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