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Construction of Environmental DNA Libraries in Escherichia coli and Screening for the Presence of Genes Conferring Utilization of 4-Hydroxybutyrate

Environmental DNA libraries from three different soil samples were constructed. The average insert size was 5 to 8 kb and the percentage of plasmids with inserts was approximately 80%. The recombinant Escherichia coli strains (approximately 930,000) were screened for 4-hydroxybutyrate utilization. T...

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Bibliographische Detailangaben
Hauptverfasser: Henne, Anke, Daniel, Rolf, Schmitz, Ruth A., Gottschalk, Gerhard
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 1999
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC99718/
https://ncbi.nlm.nih.gov/pubmed/10473393
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