Construction of Environmental DNA Libraries in Escherichia coli and Screening for the Presence of Genes Conferring Utilization of 4-Hydroxybutyrate

Environmental DNA libraries from three different soil samples were constructed. The average insert size was 5 to 8 kb and the percentage of plasmids with inserts was approximately 80%. The recombinant Escherichia coli strains (approximately 930,000) were screened for 4-hydroxybutyrate utilization. T...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Henne, Anke, Daniel, Rolf, Schmitz, Ruth A., Gottschalk, Gerhard
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	General Microbial Ecology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC99718/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10473393/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.9.3901-3907.1999
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