Identification of an NADH-Cytochrome b(5) Reductase Gene from an Arachidonic Acid-Producing Fungus, Mortierella alpina 1S-4, by Sequencing of the Encoding cDNA and Heterologous Expression in a Fungus, Aspergillus oryzae

Based on the sequence information for bovine and yeast NADH-cytochrome b(5) reductases (CbRs), a DNA fragment was cloned from Mortierella alpina 1S-4 after PCR amplification. This fragment was used as a probe to isolate a cDNA clone with an open reading frame encoding 298 amino acid residues which s...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Sakuradani, Eiji, Kobayashi, Michihiko, Shimizu, Sakayu
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1999
Schlagworte:	Genetics and Molecular Biology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC99714/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10473389/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.9.3873-3879.1999
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