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Insertion-Duplication Mutagenesis of Neisseria: Use in Characterization of DNA Transfer Genes in the Gonococcal Genetic Island
We created plasmids for use in insertion-duplication mutagenesis (IDM) of Neisseria gonorrhoeae. This mutagenesis method has the advantage that it requires only a single cloning step prior to transformation into gonococci. Chromosomal DNA cloned into the plasmid directs insertion into the chromosome...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2001
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC99525/ https://ncbi.nlm.nih.gov/pubmed/11466274 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.16.4718-4726.2001 |
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