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Function-Based Selection and Characterization of Base-Pair Polymorphisms in a Promoter of Escherichia coli RNA Polymerase-ς(70)

We performed two sets of in vitro selections to dissect the role of the −10 base sequence in determining the rate and efficiency with which Escherichia coli RNA polymerase-ς(70) forms stable complexes with a promoter. We identified sequences that (i) rapidly form heparin-resistant complexes with RNA...

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Detalhes bibliográficos
Main Authors: Xu, Jian, McCabe, Barbara C., Koudelka, Gerald B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC99504/
https://ncbi.nlm.nih.gov/pubmed/11292807
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.9.2866-2873.2001
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