Immunization with Recombinant Opc Outer Membrane Protein from Neisseria meningitidis: Influence of Sequence Variation and Levels of Expression on the Bactericidal Immune Response against Meningococci

The opc gene from Neisseria meningitidis was cloned into the pRSETA vector, and recombinant protein was expressed at high levels in Escherichia coli. The protein was readily purified by affinity chromatography and used for immunization with conventional Al(OH)(3) adjuvant or after incorporation into...

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Detalhes bibliográficos
Main Authors: Jolley, Keith A., Appleby, Lynn, Wright, J. Claire, Christodoulides, Myron, Heckels, John E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Microbial Immunity and Vaccines
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC98398/
https://ncbi.nlm.nih.gov/pubmed/11349046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.69.6.3809-3816.2001
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