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impA, a Gene Coding for an Inner Membrane Protein, Influences Colonial Morphology of Actinobacillus actinomycetemcomitans

Directed mutagenesis of a gene coding for a membrane protein of the periodontopathogen Actinobacillus actinomycetemcomitans was achieved by conjugation. The gene was disrupted by insertion of an antibiotic cassette into a unique endonuclease restriction sequence engineered by inverse PCR. The disrup...

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Bibliografiske detaljer
Main Authors: Mintz, Keith P., Fives-Taylor, Paula M.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2000
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC97753/
https://ncbi.nlm.nih.gov/pubmed/11083768
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