Construction and Characterization of Haemophilus ducreyi Lipooligosaccharide (LOS) Mutants Defective in Expression of Heptosyltransferase III and β1,4-Glucosyltransferase: Identification of LOS Glycoforms Containing Lactosamine Repeats

To begin to understand the role of the lipooligosaccharide (LOS) molecule in chancroid infections, we constructed mutants defective in expression of glycosyltransferase genes. Pyocin lysis and immunoscreening was used to identify a LOS mutant of Haemophilus ducreyi 35000. This mutant, HD35000R, prod...

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Bibliografiske detaljer
Main Authors: Filiatrault, Melanie J., Gibson, Bradford W., Schilling, Birgit, Sun, Shuhua, Munson, Robert S., Campagnari, Anthony A.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2000
Fag:
Molecular and Cellular Pathogenesis
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC97600/
https://ncbi.nlm.nih.gov/pubmed/10816485
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