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Development of a Novel In Vitro Assay (ALS Assay) for Evaluation of Vaccine-Induced Antibody Secretion from Circulating Mucosal Lymphocytes

We describe here a novel method for measuring in vitro antibody secretion from the tissue culture of human B lymphocytes in peripheral blood mononuclear cells (PBMC) after oral vaccination with a killed cholera vaccine. Enzyme-linked immunosorbent assay (ELISA) titers of the antibody secreted in the...

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Bibliografski detalji
Glavni autori: Chang, H. Sunny, Sack, David A.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2001
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC96087/
https://ncbi.nlm.nih.gov/pubmed/11329444
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.8.3.482-488.2001
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