Quantification of Bacterial Transcripts during Infection Using Competitive Reverse Transcription-PCR (RT-PCR) and LightCycler RT-PCR

Bacteria have evolved sophisticated regulatory circuits to modulate their gene expression in response to disparate environments. In order to monitor bacterial gene expression and regulation in the host, methods for direct transcript analysis from clinical specimens are needed. For most bacterial inf...

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Bibliographic Details
Main Authors: Goerke, Christiane, Bayer, Manfred G., Wolz, Christiane
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2001
Subjects:
Microbial Immunology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC96049/
https://ncbi.nlm.nih.gov/pubmed/11238208
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.8.2.279-282.2001
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