Description of a Nonlethal Herpes Simplex Virus Type 1 Glycoprotein D Deletion Mutant Affecting a Site Frequently Used for PCR

We report a herpes simplex virus type 1 mutant which failed to amplify with a commonly used glycoprotein D primer set. The virus contained a nine-base deletion in the gene's 5′ nontranslated region. The altered amplicon was clearly distinguishable on a 4% high-resolution agarose gel.

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Detalhes bibliográficos
Main Authors: Coyle, P. V., Jain, S., Wyatt, D., McCaughey, C., O'Neill, H. J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
Assuntos:
Microbial Immunology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC95871/
https://ncbi.nlm.nih.gov/pubmed/10702515
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