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Expression and Refolding of Truncated Recombinant Major Outer Membrane Protein Antigen (r56) of Orientia tsutsugamushi and Its Use in Enzyme-Linked Immunosorbent Assays

The variable 56-kDa major outer membrane protein of Orientia tsutsugamushi is the immunodominant antigen in human scrub typhus infections. The gene encoding this protein from Karp strain was cloned into the expression vector pET11a. The recombinant protein (r56) was expressed as a truncated nonfusio...

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Auteurs principaux: Ching, W.-M., Wang, H., Eamsila, C., Kelly, D. J., Dasch, G. A.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 1998
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC95611/
https://ncbi.nlm.nih.gov/pubmed/9665960
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